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pcdna3 1 zeocin mammalian expression vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna3 1 zeocin mammalian expression vector
    Pcdna3 1 Zeocin Mammalian Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+zeocin+mammalian+expression+vector/Zeocin/pm24944734__ml4004759_si_001-158-11-15
    Average 99 stars, based on 1 article reviews
    pcdna3 1 zeocin mammalian expression vector - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Generated:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Stable Transfection:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Construct:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Clone Assay:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Expressing:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Article Title: Supporting Information Towards the Development of Bivalent Ligand Probes of Cannabinoid CB1 and Orexin OX1 Receptor Heterodimers
    Article Snippet: .. Briefly, cDNA coding for OX1 (Open Biosystems) was subcloned into the pcDNA3.1/Zeocin mammalian expression vector (Life Technologies) and transfected into our CB1/RD-HGA16 cells using Fugene transfection reagent. ..

    Plasmid Preparation:

    Article Title: A cancer vaccine with dendritic cells differentiated with GM-CSF and IFNα and pulsed with a squaric acid treated cell lysate improves T cell priming and tumor growth control in a mouse model
    Article Snippet: .. The ID8-ova cell line was generated by stable transfection of ID8 cells using Express-in technology (Thermo Scientific, USA) with a construct of chicken ovalbumin (a kind gift from Dr. Y. Patterson, University of Pennsylvania) cloned in the pcDNA3.1-zeocin mammalian expression vector (Life Technologies, Carlsbad, USA). .. Tumor cells were cultured in complete DMEM (Cellgro, New York, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Life Technologies) and antibiotics (Penstrep (Gibco, Gaithersburg, USA) at 10 U/mL culture medium and normocin, (Invitrogen, Waltham, USA) at 0.1 mg/mL culture medium).

    Article Title: Supporting Information Towards the Development of Bivalent Ligand Probes of Cannabinoid CB1 and Orexin OX1 Receptor Heterodimers
    Article Snippet: .. Briefly, cDNA coding for OX1 (Open Biosystems) was subcloned into the pcDNA3.1/Zeocin mammalian expression vector (Life Technologies) and transfected into our CB1/RD-HGA16 cells using Fugene transfection reagent. ..

    Transfection:

    Article Title: Supporting Information Towards the Development of Bivalent Ligand Probes of Cannabinoid CB1 and Orexin OX1 Receptor Heterodimers
    Article Snippet: .. Briefly, cDNA coding for OX1 (Open Biosystems) was subcloned into the pcDNA3.1/Zeocin mammalian expression vector (Life Technologies) and transfected into our CB1/RD-HGA16 cells using Fugene transfection reagent. ..



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